Journal: Diabetologia

Article Title: Treatment with a β-2-adrenoceptor agonist stimulates glucose uptake in skeletal muscle and improves glucose homeostasis, insulin resistance and hepatic steatosis in mice with diet-induced obesity

doi: 10.1007/s00125-020-05171-y

Figure Lengend Snippet: In vitro and acute in vivo effects of clenbuterol. ( a ) Clenbuterol stimulation of L6 myotubes induced glucose uptake in a dose-dependent manner; n = 5–8; data were analysed by one-way ANOVA with Dunnett’s multiple comparison test. ( b , c ) Stimulation of L6 myotubes stably expressing GLUT4myc with 1 μmol/l clenbuterol induces GLUT4 translocation as quantified in ( c ); n = 3; data were analysed with unpaired two-tailed Student’s t test. Scale bars, 50 μm. ( d ) In vivo glucose uptake in gastrocnemius muscle of chow-fed mice treated with 1 mg/kg clenbuterol for 1 h; n = 5–6; data were analysed with unpaired two-tailed Student’s t test. ( e , f ) Acute effects of clenbuterol on blood glucose ( e ) and plasma insulin ( f ); n = 6 (diet-induced obesity was developed in C57Bl/6N mice maintained at 30°C and on HFD for 7 months; mice were fasted in the morning for 6 h prior to clenbuterol, glucose or saline i.p. injection); data were analysed by two-way ANOVA with Dunnett’s multiple comparison test. In all graphs: * p < 0.05, ** p < 0.01, *** p < 0.001 vs vehicle (in e and f vs vehicle at the same time point)

Article Snippet: L6 rat myoblasts and L6 myoblasts stably expressing GLUT4myc were purchased from KeraFast (ESK201 and ESK202), where they were tested for mycoplasma.

Techniques: In Vitro, In Vivo, Stable Transfection, Expressing, Translocation Assay, Two Tailed Test, Injection